Calcium and magnesium are concentration-dependent pro- and anticoagulant cofactors, and magnesium behaves similarly to calcium in the presence of citrate. The authors hypothesized that magnesium can cause clot formation (primary objective) when mixed with coagulation factor-containing blood products diluted with different crystalloids in a rapid- infuser reservoir. A secondary objective was the observation of any infuser alarms and stops in the event of clotting.

An experimental in vitro study with blood products, crystalloids, magnesium, and calcium in a rapid infuser with a reservoir using a closed-loop system.

Anesthesia research laboratory at an urban academic tertiary medical center

Not applicable.

Exposure of fresh frozen plasma (FFP) and packed red blood cells alone (control) or in combination with either normal saline (NS), lactated Ringer’s solution (LR), or Plasma-Lyte A (PL) to increasing concentrations of magnesium sulphate (MgSO₄) up to 1 g. After each incremental MgSO₄ change, the authors applied a specific pump-flow sequence in a closed-loop system with a rapid-infuser reservoir, and if no clot was observed, the authors incrementally added calcium chloride (CaCl₂) up to 1 g.

Observation of macroscopic clot and time to event, as well as occurrence and type of any pump alarms or stops. LR experiments resulted in clot observation in the reservoir by a dedicated observer after MgSO₄ 275 ± 206 mg (95% confidence interval [CI], 9-541). Adding MgSO₄ 1 g in the NS, PL, or the control experiments did not result in clot observation. Only when CaCl₂ 166.7 ± 51.64 mg (95% CI, 112.0-22.01) was added to the combination of blood products alone or mixed with NS and PL, clotting occurred. The mean FFP volume was 281 ± 48.6 mL (range, 204-340 mL) and was not different between groups (p = 0.44). Pump alarms and stops were inconsistent.

The addition of magnesium to a combination of LR with coagulation factor- containing blood products consistently resulted in a visible blood clot in the rapid-infuser reservoir in the authors’ experimental setup. In addition to MgSO₄ 1 g in the control, NS, and PL experiments, CaCl₂ is needed before a clot can be observed.